Tool assembly: Plant Genomics
What is the plant genomics tool assembly?
The plant genomics tool assembly is a toolkit for managing plant genomics and genotyping data throughout their life cycle, with a particular focus on ensuring traceability of the biological material to enable interoperability with plant phenotyping data. To enable this, the same persistent identifiers must be used in both the genotyping and phenotyping experiments. It is recommended that the biological plant material is accurately described using rich metadata and stored in a central repository. The tool assembly also provides guidance on how users should structure their analysis results in the form of VCF files to achieve a higher degree of interoperability.
Who can use the plant genomics tool assembly?
This tool assembly can be used by any researcher producing plant genomic or genotyping data interested in ensuring their data complies with the FAIR principles.
How can you access the plant genomics tool assembly?
All the components of this tool assembly are publicly available, but most require registration. So anyone can access the tool assembly provided they register for each tool that requires it.
For what purpose can you use the plant genomics tool assembly?
Figure 1. The plant genomics tool assembly.
Metadata collection and tracking
Accurate documentation of the plant biological materials and samples is critical for interoperability, and should comply with the MIAPPE standard. This information should be submitted to BioSamples, with MIAPPE compliance validated using BioSamples’ plant-miappe.json template available on the sample validation page. Submission of sample descriptions to BioSamples can be done as early as the data collection stage, but at the latest, must acompany submission of the genomic data to the European Nucleotide Archive (ENA) or of genotyping data to the European Variation Archive (EVA). The complete timeline for submitting plant biological material to BioSamples and resulting genotyping experiment results to ENA and EVA should look like this:
- Register plant biological material information to BioSamples
- Submit Sequencing reads to ENA (using BioSamples IDs to identify material)
- Check if used reference genome assembly is INSDC available (GCF / GCA accesion number available)
- If yes proceed to submit VCF at step 4, if no proceed to step 3 b
- Submit reference genome assembly to INSDC (NCBI Genbank / EBML-EBI ENA / DDBJ) and wait until accession number is issued, then proceed to step 4
- Submit VCF file to EVA (using BioSamples IDs to identify material, GCF/GCA accession for the reference genome assembly)
e!DAL-PGP, FAIRDOM-SEEK instances such as FAIRDOMHub or Recherche Data Gouv can be used to manage and share experimental metadata, as well as data.
Data processing and analysis
Reference genomes for genome assembly and annotation should be obtained from ENSEMBL Plants or PLAZA, if available. Genetic variant data must be produced in the VCF format, and validated using the EVA vcf-validator (https://github.com/EBIvariation/vcf-validator). Please note to only use identifiers of sequences that match the reference genome assembly identifiers. In order to ensure interoperability of VCF files, the VCF meta-information lines should be used: see the Plant sciences page for more details.
Data sharing and publishing
All sequencing data collected in plant genotyping experiments should be submitted to ENA together with metadata compliant to the GSC MIxS plant associated checklist. Final results of such studies in the form of VCF files should be submitted to EVA. Additionally, supplemental data complementing these two data types is encouraged to be submitted to e!DAL-PGP or Recherche Data Gouv.
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Anne-Françoise Adam-Blondon
INRAE / ELIXIR-FR
Cyril Pommier
INRAE / ELIXIR-FR
Editor
Daniel Faria
University of Lisbon / ELIXIR-PT
Erwan Le Floch
INRAE / ELIXIR-FR
Paulette Lieby
Institut Français de Bioinformatique / ELIXIR-FR
Sebastian Beier